Composite

Part:BBa_K5226077:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-10-01)


gRNA-△cysNC-key


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1852
    Illegal XbaI site found at 98
    Illegal PstI site found at 1474
    Illegal PstI site found at 2047
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1852
    Illegal NheI site found at 765
    Illegal PstI site found at 1474
    Illegal PstI site found at 2047
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1852
    Illegal BamHI site found at 92
    Illegal BamHI site found at 1002
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1852
    Illegal XbaI site found at 98
    Illegal PstI site found at 1474
    Illegal PstI site found at 2047
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1852
    Illegal XbaI site found at 98
    Illegal PstI site found at 1474
    Illegal PstI site found at 2047
    Illegal NgoMIV site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

The sgRNA sequence was obtained using the RNAfold Webserver, while the left and right homology arms of the target gene were generated through PCR amplification. The sequence originates from the Halomonas sp. TD01 strain, which can be found on NCBI (https://www.ncbi.nlm.nih.gov/nuccore/OV350343).

References